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Lab Techniques

Definitions:
ion exchange chromatography, affinity chromatography, genetically tagged protein, genetically tagged protein, photoactivation, Fluorescence Recovery After Photobleaching, observing/ meausring activity, genetically tagged protein, tracking location and movement, protein purification/ separation, Fluorescence Resonance Energy Transfer, protein purification/ separation, hybridomas language , gel filtration chromatography web tool , hybrid cells, observing/ meausring activity web tool ,

Answers:
centrifugation, histidine tag , separates by size, separates by highly specific interaction: enzyme and substrate , immortal life, chromatography, separates by charge, allows the followin of complex intracellular processes, bleach kills fluoresence, bleach with laser and observe movement , have a finite life, Fluorescence Recovery After Photobleaching, Fluorescence Resonance Energy Transfer, shows intereactions, if blue no, if green then yes , glutathion tag, green fluorescent protein, photoactivation,